Measurement of NAD/NADH Ratio

NAD/NADH ratio was measured with NAD/NADH assay kit purchased from Abcam. Briefly, HUH6 cells were cultured with or without 5 μM CQ for 96 h. Cells were lysed and processed following manufacturer's instructions as described (49 (link)). The reaction was allowed to develop for 1.5 h, and then absorbance was measured at 450 nm with Multiskan FC microplate reader (Thermo Fisher Scientific, Vantaa, Finland). NAD/NADH ratio was calculated using normalized concentrations by equation ([NADtotal—NADH])/[NADH].

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Eloranta K., Cairo S., Liljeström E., Soini T., Kyrönlahti A., Judde J.G., Wilson D.B., Heikinheimo M, & Pihlajoki M. (2020). Chloroquine Triggers Cell Death and Inhibits PARPs in Cell Models of Aggressive Hepatoblastoma. Frontiers in Oncology, 10, 1138.

Publication 2020

NAD/NADH assay kit Multiskan FC microplate reader

Assay Cells Nadh

Corresponding Organization :

Other organizations : University of Helsinki, Helsinki University Hospital, Karolinska Institutet, St. Louis Children's Hospital

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Variable analysis

independent variables

Treatment with 5 μM CQ

dependent variables

NAD/NADH ratio

control variables

HUH6 cells

controls

Cells cultured without 5 μM CQ (negative control)

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