Flavonoid Pre-Treatment Effects on Protein Expression

MDA-MB231 cells and LECs were grown in T-25 tissue culture flasks (Nunc, Roskilde, Denmark) to 80% confluence and then pre-treated with the flavonoids for 0.5, 1, 2, and 4 h. Then, cells were processed for SDS gel electrophoresis and Western blotting as described before (Nguyen et al., 2015 (link)). Chemo-luminescence was developed by Amersham ECL prime Kit (GE Healthcare, Freiburg, Germany) and detected using a Lumi-Imager F1 Workstation (Roche, Basel, Switzerland). Densitometry of the Western blots was analyzed with the Image-J software (National Institutes of Health, Bethesda, MD, United States).

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Hong J., Fristiohady A., Nguyen C.H., Milovanovic D., Huttary N., Krieger S., Hong J., Geleff S., Birner P., Jäger W., Özmen A., Krenn L, & Krupitza G. (2018). Apigenin and Luteolin Attenuate the Breaching of MDA-MB231 Breast Cancer Spheroids Through the Lymph Endothelial Barrier in Vitro. Frontiers in Pharmacology, 9, 220.

Publication 2018

T-25 tissue culture flasks Amersham ECL prime Kit Lumi-Imager F1 Workstation

Cells Densitometry Electrophoresis Flavonoids Luminescence Tissue Western blots

Corresponding Organization : University of Vienna

Other organizations : Nanjing Medical University, Haluoleo University, 117th Hospital of People's Liberation Army, Adnan Menderes University

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Variable analysis

independent variables

Flavonoids

dependent variables

Protein expression (measured by Western blotting)

control variables

MDA-MB231 cells and LECs were grown in T-25 tissue culture flasks (Nunc, Roskilde, Denmark) to 80% confluence
Cells were processed for SDS gel electrophoresis and Western blotting as described before (Nguyen et al., 2015)
Chemo-luminescence was developed by Amersham ECL prime Kit (GE Healthcare, Freiburg, Germany) and detected using a Lumi-Imager F1 Workstation (Roche, Basel, Switzerland)
Densitometry of the Western blots was analyzed with the Image-J software (National Institutes of Health, Bethesda, MD, United States)

controls

No specific positive or negative controls were mentioned in the input.

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