Immune Cell Culture and Mouse Experiments

Both types of mouse lymphocytes used in the experiment, namely DC2.4 cells and E.G7-OVA cells, were purchased from the American Type Culture Collection (ATCC). BALB/c mice and C57BL/6 mice (SPF, female, 6–8 weeks of age) were purchased from Beijing HFK Bioscience and Hunan SJA Laboratory Animal Co. (China), respectively. The animal experimental protocols were approved by the Laboratory Animal Welfare and Ethics Committee of the Third Military Medical University and performed according to the Guide for the Care and Use of Laboratory Animals (27 (link)); professional technicians, skilled operation of equipment, and suitable experimental conditions for the animals were ensured in order to minimize pain experienced by the mice.