Antibacterial Metabolites Profiling

The inhibitory metabolites produced by the bacteria tested were inferred and detected using a well-diffusion technique^{10 (link)}. The chosen strains were grown in overnight cultures on MRS agar for 24 h at 37 °C. The study's indicator microorganisms, which were obtained from the Persian Type Culture Collection (PTCC), were Streptococcus sanguinis PTCC 1449, Streptococcus salivarius PTCC 1448, Streptococcus sobrinus PTCC 1601, Yersinia enterocolitica ATCC 23715, Pseudomonas aeruginosa PTCC 1181, Staphylococcus aureus ATCC 25923, Streptococcus mutans PTCC 1683, Bacillus subtilis ATCC 19652, Klebsiella pneumoniae PTCC 1053, Listeria monocytogenes ATCC 13932, Shigella flexneri PTCC 1234, and Escherichia coli PTCC 1276. Half a McFarland of indicator bacteria (1.5 10⁸ CFU mL⁻¹) was poured into various appropriate media, and wells were cut into plates. The plates were then incubated overnight at 37 °C with each well filled with 50 µL filtered supernatant, and a digital caliper was used to estimate the inhibitory zone around the wells.