Propagation of RVFV Strain MP-12 in Vero-76 Cells

RVFV strain MP-12 propagation follows previously published protocols [47 (link)]. Briefly, it was propagated on Vero-76 cells in Dulbecco’s Modified Eagle’s Medium (DMEM, #DMEM-HXA, Capricorn Scientific GmbH, Ebsdorfergrund, Germany)/2% fetal bovine serum (FBS; #FBS-HI-12A FBS, Capricorn Scientific GmbH, Ebsdorfergrund, Germany) under environmental conditions of 37 °C and a CO₂ content of 5%. Supernatant of infected cells was harvested after three days, with cells showing a cytopathic effect in 80% of cells, and titers were evaluated in a TCID₅₀ assay and calculated according to Spearman and Kaerber [48 (link),49 (link)].