Normal and polycystic human cholangiocytes were isolated according to a novel protocol recently described by our group.15 (link) Normal cholangiocytes were isolated from bordering tissue samples obtained during surgical dissection of a local hepatic adenoma in a female patient; only tissue pieces identified as normal by an experienced pathologist were employed.15 (link) In contrast, polycystic human cholangiocytes were isolated from a female patient who had undergone hepatorenal transplant for advanced ADPKD at the Clinic University of Navarra. Cholangiocyte primary cultures from normal and polycystic kidney (PCK) male rats were also employed.16 (link)17 (link)
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Urribarri A.D., Munoz-Garrido P., Perugorria M.J., Erice O., Merino-Azpitarte M., Arbelaiz A., Lozano E., Hijona E., Jiménez-Agüero R., Fernandez-Barrena M.G., Jimeno J.P., Marzioni M., Marin J.J., Masyuk T.V., LaRusso N.F., Prieto J., Bujanda L, & Banales J.M. (2014). Inhibition of metalloprotease hyperactivity in cystic cholangiocytes halts the development of polycystic liver diseases. Gut, 63(10), 1658-1667.
Publication 2014
AdpkdDissection Female Hepatic adenoma Human Male Pathologist Patient Polycystic kidney Rats Surgical Tissue Transplant
Corresponding Organization : Biogipuzkoa Health Research Institute
Other organizations :
Universidad de Navarra, University of the Basque Country, Instituto de Salud Carlos III, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Ikerbasque, Universidad de Salamanca, Instituto de Investigación Biomédica de Salamanca, Marche Polytechnic University, Mayo Clinic, WinnMed
Cholangiocyte primary cultures from normal and polycystic kidney (PCK) male rats
dependent variables
Not explicitly mentioned
control variables
Only tissue pieces identified as normal by an experienced pathologist were employed for isolating normal cholangiocytes
controls
Positive control: Normal cholangiocytes
Negative control: Cholangiocyte primary cultures from polycystic kidney (PCK) male rats
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