Rainbow trout utilized as gamete donors originated from the spring spawning broodstock raised in the Department of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, Poland. The number of dams and sires used in the research was determined to meet the requirements for the statistical analysis and it was based on the previous studies concerning the chromosomes set manipulations in fish [12 (link),13 (link),15 (link)]. Semen was taken from four males, pooled and kept in the plastic box. The motility of the spermatozoa was analyzed under microscope (Nikon Eclipse E 2000). Sperm was stored in the fridge until use. Four randomly selected females (F1–4) were stripped and the eggs were collected into separate plastic containers. After the quality examination, the eggs were kept at 10 °C until further manipulations. Fin clips from each gamete donors were stored in 96% ethanol for the molecular analysis. Approximately 240 eggs from each female were inseminated using untreated spermatozoa to establish control groups (Fc1–4) for the gynogenetic variants of the experiment.
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