Trypticase soy broth was procured from Fisher Scientific (Hampton, NH, USA). Fetal bovine serum (FBS) and gentamicin (catalog no. 15750060) were purchased from Gibco-BRL (Grand Island, NY, USA). α-Minimal essential medium (α-MEM), anti-α-actin (A2228) antibody, rosiglitazone, polyinosinic-poly(C) [poly(I·C)], and lysostaphin (L7386) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Macrophage colony-stimulating factor (M-CSF), in the form of CMG 14-12 supernatant, and glutathione S-transferase RANKL (GST-RANKL) were prepared as previously described (33 (link)). Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn, CA, USA). Anti-NFATc1 (7AG) antibody was obtained from Santa Cruz Biotechnology (Dallas, TX, USA), and anti-histone H3 (96C10) was obtained from Cell Signaling Technology (Beverly, MA, USA). Murine recombinant IL-4 and IFN-γ were purchased from Peprotech (Rocky Hill, NJ, USA).
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