We established primary CAFs (CAF1 and CAF2) from surgically resected PC tissues at Gunma University using a previously described method.
16 (link) The resected PC samples were used in accordance with the Helsinki Declaration and Institutional Review Board of Gunma University (approval number: 2016‐118) after obtaining written informed consent. We used the CAFs between passage numbers 4 and 8. The human PC cell lines, Suit2 and BXPC3, were obtained from the JCRB Cell Bank and SW1990 was obtained from the ATCC. PANC1 and the human pancreatic stellate cell line hPSC5, derived from PC, was provided by RIKEN BRC. The human HSC line LX‐2 was purchased from Millipore. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (Thermo Fisher Scientific) and maintained at 37°C in a humidified incubator with 5% CO2 atmosphere.
16 (link) The resected PC samples were used in accordance with the Helsinki Declaration and Institutional Review Board of Gunma University (approval number: 2016‐118) after obtaining written informed consent. We used the CAFs between passage numbers 4 and 8. The human PC cell lines, Suit2 and BXPC3, were obtained from the JCRB Cell Bank and SW1990 was obtained from the ATCC. PANC1 and the human pancreatic stellate cell line hPSC5, derived from PC, was provided by RIKEN BRC. The human HSC line LX‐2 was purchased from Millipore. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin–streptomycin (Thermo Fisher Scientific) and maintained at 37°C in a humidified incubator with 5% CO2 atmosphere.
