The CBS/CSE inhibitor AOAA (O-(carboxymethyl)hydroxylamine hemihydrochloride) [9 (link)] was purchased from Sigma-Aldrich (Burlington, MA, United States), the 3-MST inhibitor HMPSNE (2-[(4-hydroxy-6-methylpyrimidin-2-yl)sulfanyl]-1-(naphthalen-1-yl)ethan-1-one) [10 (link)] was purchased from MolPort (Riga, Latvia). The fluorescent H2S probe 7-azido-4-methylcoumarin (AzMC) was obtained from Sigma-Aldrich (Burlington, MA, USA).
Rabbit monoclonal anti-CBS (D8F2P), anti-ATP citrate lyase (ACLY) (D1 × 6P), anti-β-catenin (D10A8), anti-mouse IgG HRP-linked antibody (#7076) and rabbit polyclonal anti-phospho-ACLY (Ser455) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-β-actin (AC-15) and rabbit polyclonal anti-SQR (HPA017079) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Anti-rabbit IgG (H+L) cross-adsorbed secondary antibody-HRP (#31458) was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal anti-3-MST (ab224043), anti-CSE (ab151769), anti-TST (ab231248) and rabbit monoclonal anti-ETHE1 (ab174302) were purchased from Abcam (Cambridge, UK).
Rabbit monoclonal anti-CBS (D8F2P), anti-ATP citrate lyase (ACLY) (D1 × 6P), anti-β-catenin (D10A8), anti-mouse IgG HRP-linked antibody (#7076) and rabbit polyclonal anti-phospho-ACLY (Ser455) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse monoclonal anti-β-actin (AC-15) and rabbit polyclonal anti-SQR (HPA017079) were obtained from Sigma-Aldrich (Saint Louis, MO, USA). Anti-rabbit IgG (H+L) cross-adsorbed secondary antibody-HRP (#31458) was purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Rabbit polyclonal anti-3-MST (ab224043), anti-CSE (ab151769), anti-TST (ab231248) and rabbit monoclonal anti-ETHE1 (ab174302) were purchased from Abcam (Cambridge, UK).
Free full text: Click here
