Protocol detail

Drosophila Larval Neurogenesis Assay

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Flies were cultured with standard food in plastic vials at 25 °C. 3^rd instar wandering larvae were analyzed in all the experiments. Fly strains are used as follows: nub-gal4 (Bloomington Drosophila Stock Center [BDSC] #42699), rn-gal4^GAL4-5 (BDSC #7405), UAS-CD8-PARP-Venus (gift from Yasushi Hiromi)^{50 (link)}, UAS-bsk-RNAi (National Institute of Genetics [NIG] #5680R-2), slit² (Drosophila Genomics and Genetic Resources [DGGR] #106948)^{51 (link)}, UAS-slit-RNAi (BDSC #31468), robo2² (DGGR #106843)^{42 (link)}, UAS-robo2-RNAi (BDSC #34589), slit⁰⁵²⁴⁸ (slit-lacZ, BDSC #12189)^{52 (link),53 (link)}, UAS-slit (gift from Tom Kidd), UAS-robo2-HA (gift from Talia Volk), wg¹ (BDSC #2978)⁵⁴, wg^SP-1 (BDSC #405)^{55 ,56}, dpp^d6 (DGGR #106644)^{57 (link)}, dpp^s11 (DGGR #106646)^{57 (link)}, dpp^hr92 (DGGR #106649)^{58 (link)}, TRE-DsRed (BDSC #59012)^{48 (link)}, P{PZ}dpp¹⁰⁶³⁸ (dpp-lacZ, BDSC #12379)^{52 (link)}.

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Iida C., Ohsawa S., Taniguchi K., Yamamoto M., Morata G, & Igaki T. (2019). JNK-mediated Slit-Robo signaling facilitates epithelial wound repair by extruding dying cells. Scientific Reports, 9, 19549.

Publication 2019

nub-gal4 TRE-DsRed dpp-lacZ

Cultured food Drosophila Lacz Larvae Resources Rnai Strains

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Variable analysis

independent variables

Nub-gal4
Rn-gal4^GAL4-5
UAS-CD8-PARP-Venus
UAS-bsk-RNAi
Slit^2
UAS-slit-RNAi
Robo2^2
UAS-robo2-RNAi
Slit^05248 (slit-lacZ)
UAS-slit
UAS-robo2-HA
Wg^SP-1
Dpp^d6
Dpp^s11
Dpp^hr92
TRE-DsRed
P{PZ}dpp^10638 (dpp-lacZ)

dependent variables

3rd instar wandering larvae

control variables

Flies were cultured with standard food in plastic vials at 25 °C

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