Cell Line Mycoplasma Screening Protocol

All cell lines used in this study were tested for mycoplasma using a PCR-based method (24 (link)). This testing was performed upon obtaining the cells and every 8–12 weeks to confirm that all experiments were performed in uninfected cells. HEK293T cells (American Type Culture Collection, ATCC) were cultured in DMEM supplemented with 10% FBS and l-glutamine. NK92mi cells were grown in X-vivo medium (Lonza) with 10% human serum. YTS cells were cultured in IMDM supplemented with 12.5% FBS. Human B lymphoblastoid 721.221 cells and ovarian carcinoma SKOV-3 cells were cultured in complete RPMI 1640 medium (Gibco, Thermo Fisher Scientific). Patient-derived dermal fibroblasts were obtained after IRB approval and informed consent, as previously described (10 (link)). Fibroblasts were cultured in DMEM supplemented with 10% FBS. Etoposide (MilliporeSigma) was used as a 1-mM solution in DMSO. Poly(dA:dT) (1 mg/mL, InvivoGen) was transfected using LyoVec (InvivoGen) following the manufacturer’s instructions.