Primary antibodies used for immunofluorescence were against MYOD (1:20; Santa Cruz Biotechnology), MYHC (1:20; MF-20, DSHB), PERILIPIN A/B (1:100; Sigma-Aldrich), Sca-1-FITC (1:100; eBioscience), and α7-Integrin-PE (1:100; eBioscence). Oil red O was used to stain lipids, as described in Mozzetta et al. (2013) (link). Further experimental details are provided in the Supplemental Material.
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Immunofluorescence Analysis of Muscle Stem Cells
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Corresponding Organization :
Other organizations : Fondazione Santa Lucia, Istituti di Ricovero e Cura a Carattere Scientifico, Sanford Burnham Prebys Medical Discovery Institute, European Institute of Oncology, University of Toronto, Hospital for Sick Children, University of California, San Francisco, University of Milan
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- Primary antibodies used for immunofluorescence were against MYOD (1:20; Santa Cruz Biotechnology), MYHC (1:20; MF-20, DSHB), PERILIPIN A/B (1:100; Sigma-Aldrich), Sca-1-FITC (1:100; eBioscience), and α7-Integrin-PE (1:100; eBioscence)
- Immunofluorescence staining patterns of MYOD, MYHC, PERILIPIN A/B, Sca-1, and α7-Integrin
- Oil red O staining to stain lipids, as described in Mozzetta et al. (2013)
- No positive or negative controls were explicitly mentioned.
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