Fourier-transformed infrared (FTIR) spectrophotometry was employed to determine chemical changes among the extracts and their fractions [32 (link)]. FTIR spectra were recorded in a Bruker FT-MIR model Vertex 70 V spectrometer. Wave number range was set in the range of 800 to 4000 cm−1. Samples were blended with KBr and compressed into disks. FTIR spectra analysis was carried out by using Omnic 7.1 software (Thermo Fisher Scientific, USA).
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