Histological and Immunohistochemical Analysis of Colonic Specimens

Colonic specimens were prepared and analyzed by pathologists at the City of Hope Pathology Core Lab. Histology and immunohistochemical staining procedures were described previously^{29 (link)}. The antibodies used were as follows: BrdU (DAKO, Cat#: M0744), Ki67 (DAKO, Cat#: M7204), and P-Stat3 Tyr705 (Cell Signaling Technology, Cat#: 9145). TUNEL staining was performed using the Cell Death Detection Kit from Roche (Cat#: 11684795910), according to the manufacturer’s instructions. Standards for tumor grading, epithelial damage score and inflammatory cell infiltration score are described in Tables S1, S2 and S3^{66 (link)}.

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Ma X., Meng Z., Jin L., Xiao Z., Wang X., Tsark W.M., Ding L., Gu Y., Zhang J., Kim B., He M., Gan X., Shively J.E., Yu H., Xu R, & Huang W. (2017). CAMK2γ in Intestinal Epithelial Cells Modulates Colitis-Associated Colorectal Carcinogenesis via Enhancing STAT3 Activation. Oncogene, 36(28), 4060-4071.

Publication 2017

BrdU P-Stat3 Tyr705 Cell Death Detection Kit

Antibodies Brdu Cell Cell death Colonic Inflammatory Pathologists Stat3 Tunel

Corresponding Organization :

Other organizations : City Of Hope National Medical Center, Zhejiang University

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Variable analysis

independent variables

Colonic specimens preparation and analysis by pathologists at the City of Hope Pathology Core Lab

dependent variables

Histology and immunohistochemical staining outcomes using BrdU, Ki67, and P-Stat3 Tyr705 antibodies
TUNEL staining using the Cell Death Detection Kit from Roche

control variables

Histology and immunohistochemical staining procedures described previously in reference 29
Standards for tumor grading, epithelial damage score, and inflammatory cell infiltration score described in referenced supplementary tables (Tables S1, S2, and S3)

controls

Positive and negative controls not explicitly mentioned

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