Establishing Drug-Resistant Cell Lines

Human Intestinal Epithelial Cells (HIEC) and Human colorectal adenocarcinoma cells (HCT116), purchased from American Type Culture Collection, were cultured in 1640 medium containing 10% fetal bovine serum, 100 units/mL penicillin, and 0.1 mg/mL streptomycin, and the medium was changed every 2 days. The cell line of HCT116 was obtained from American Type Culture Collection (USA). While, the OX-resisted cell line of HCT116/DR was obtained from Shanghai Meixuan Biotechnology Co., Ltd., which was establishment on HCT116 (American Type Culture Collection, USA) by a drug-induced method with gradually increased OX concentration. The basic principle of building drug-resistant cell lines in vitro by increasing drug concentration method is that long-term contact with low-dose drugs causes changes in the drug chemical process of the cell itself, and the appearance of Pgp glycoprotein (P-glycoprotein) on the cell membrane makes the cell gradually tolerant to drugs [37 (link), 38 (link)].