Multivariate Characterization of Mesenchymal Stem Cells

Several in vitro assays were performed to assess the cellular activities of MSCs. An MTT assay (Sigma-Aldrich) was used to assess cell proliferation, and a colony forming unit-fibroblast (CFU-F) assay was used to assess self-renewal. Multipotency (in vitro differentiation into chondrogenic, osteogenic, or adipogenic lineages) and transwell migration in response to platelet-derived growth factor (PDGF; 10 ng/mL PDGF-AA, R&D Systems, Minneapolis, MN, USA) were also assessed. Angiogenesis was quantified using Matrigel, and in vitro anti-inflammation was analyzed as described previously^{8 (link)–10 (link),20 (link),21 (link)}. The digital images generated in these assays were assessed quantitatively using Image-Pro 5.0 software (Media Cybernetics, Rockville, MD, USA).

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Ju H., Yun H., Kim Y., Nam Y.J., Lee S., Lee J., Jeong S.M., Heo J., Kwon H., Cho Y.S., Jeong G., Ryu C.M, & Shin D.M. (2023). Activating transcription factor-2 supports the antioxidant capacity and ability of human mesenchymal stem cells to prevent asthmatic airway inflammation. Experimental & Molecular Medicine, 55(2), 413-425.

Publication 2023

MTT assay PDGF-AA Image-Pro 5.0 software

Adipogenic Angiogenesis Anti inflammation Assays Cell proliferation Chondrogenic Fibroblast Matrigel Osteogenic Pdgf Pdgf aa

Corresponding Organization : Asan Medical Center

Other organizations : University of Ulsan, Ulsan College, SK Group (South Korea)

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Variable analysis

independent variables

Platelet-derived growth factor (PDGF; 10 ng/mL PDGF-AA)

dependent variables

Cell proliferation (assessed by MTT assay)
Self-renewal (assessed by colony forming unit-fibroblast (CFU-F) assay)
Multipotency (in vitro differentiation into chondrogenic, osteogenic, or adipogenic lineages)
Migration in response to PDGF (assessed by transwell migration)
Angiogenesis (quantified using Matrigel)
Anti-inflammation (analyzed as described previously)

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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