Astrocyte Hypoxia Exposure Conditions

In mimicking the hypoxia condition, a chamber (Model C374, BioSpherix, Union St Parish, NY USA) with adjustable mixture of oxygen and carbon dioxide gasses (Linde Malaysia Sdn. Bhd., Malaysia) was used. The astrocyte cells were plated on 12-well culture plates (Cat no: 0030721012, Eppendorf AG, Harmburg, Germany) with a 15 mm cover slip (EMS, Pennsylvania USA) and then were exposed to hypoxic condition via hypoxia chamber. At first, the cells were exposed to hypoxia at various time points; 5 min, 10 min, 15 min, 20 min, 25 min and 30 min at hypoxia, 3% O₂. Then, 15 min was chosen as the time point. The cells were then exposed to different oxygen concentrations; 15% O₂, 10% O₂, 5% O₂ and 3% O₂ in 15 min (16 (link), 17 (link)).