Lymphocytes were incubated with medium 199 (control) or with the following human hormones: adiponectin (Sigma, St. Louis, MO, USA) at 100 ng/mL, leptin (Thermo Fisher, Carlsbad, CA, USA) at 100 ng/mL, melatonin (Sigma, St. Louis, MO, USA) at 100 ng/mL, adiponectin + leptin at 100 ng/mL, and melatonin + leptin at 100 ng/mL. The stimuli were endotoxin-free. The hormone concentrations were determined according to previous research with mononuclear cells from human colostrum taken from eutrophic and obese puerperal women [6 (link),7 (link)].
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