Genetic Markers for Cardiometabolic Traits

We selected 96 previously identified GWAS/literature reported risk SNPs for cardio-metabolic traits (Kathiresan et al., 2008 (link); Kooner et al., 2011 (link); Willer et al., 2008 (link))(http://www.genome.gov/gwastudies) as described in detail in our ongoing unpublished work (Farook et al.) for this study. Genomic DNA was extracted from blood samples collected from the MA children who participated in the SAFARI study, using Qiagens’s QIAmp DNA 96 BLOOD KIT or QIAmp DNA BLOOD MINIKIT according to the manufacturer’s protocol. Genotyping of the 96 SNPs was done using the Illumina’s Goldengate Veracode Assay per the manufacturer’s protocol (Illumina, San Diego, CA; www.illumina.org).

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Arya R., Farook V.S., Fowler S.P., Puppala S., Chittoor G., Resendez R.G., Mummidi S., Vanamala J., Almasy L., Curran J.E., Comuzzie A.G., Lehman D.M., Jenkinson C.P., Lynch J.L., DeFronzo R.A., Blangero J., Hale D.E., Duggirala R, & Diego V.P. (2018). Genetic and Environmental (Physical Fitness and Sedentary Activity) Interaction Effects on Cardiometabolic Risk Factors in Mexican American Children and Adolescents. Genetic epidemiology, 42(4), 378-393.

Publication 2018

Goldengate Veracode Assay

Assay Blood Children Genomic Gwas Snps

Corresponding Organization : The University of Texas Rio Grande Valley

Other organizations : The University of Texas Health Science Center at San Antonio, Atrium Health Wake Forest Baptist, Geisinger Medical Center, Pennsylvania State University, University of Pennsylvania, Obesity Society, Penn State Milton S. Hershey Medical Center

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Variable analysis

independent variables

96 previously identified GWAS/literature reported risk SNPs for cardio-metabolic traits

dependent variables

Genotyping of the 96 SNPs

control variables

Genomic DNA extracted from blood samples collected from the MA children who participated in the SAFARI study, using Qiagens's QIAmp DNA 96 BLOOD KIT or QIAmp DNA BLOOD MINIKIT according to the manufacturer's protocol
Genotyping of the 96 SNPs was done using the Illumina's Goldengate Veracode Assay per the manufacturer's protocol (Illumina, San Diego, CA; www.illumina.org)

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