Caspase-3 Activity Assay Protocol

A caspase-3 activity assay kit (BioVision, China) was used to measure caspase-3 activity. First, the collected cells were processed in lysis buffer containing a protease inhibitor cocktail (Merck, Darmstadt, Germany) for 30 min. After centrifugation, the supernatant containing the total protein samples was prepared for cell lysates. Protein samples (5 µL) were treated with Ac-DEVD-AMC agent and read using a Polarstar spectrofluorimeter at 460 nm [21 (link)].

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Tang W., Zhang L., Li J, & Guan Y. (2022). AFAP1 antisense RNA 1 promotes retinoblastoma progression by sponging microRNA miR-545-3p that targets G protein subunit beta 1. Bioengineered, 13(3), 5638-5652.

Publication 2022

caspase-3 activity assay kit protease inhibitor cocktail

Ac devd amc Assay Buffer Caspase 3 Cell Centrifugation Protease inhibitor Protein

Corresponding Organization :

Other organizations : First Affiliated Hospital of Chengdu Medical College

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Variable analysis

independent variables

Treatment of protein samples with Ac-DEVD-AMC agent

dependent variables

Caspase-3 activity

control variables

Protein concentration in cell lysates
Assay conditions (e.g., temperature, time, reagents)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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