Echocardiography and Cardiomyocyte Mechanics

The heart performance was measured by Echocardiography in all mice 48 h after LPS injection (14.0 MHz transducer, Sequoia C512; Acuson, Siemens, Erlangen, Germany). The cardiomyocytes mechanical properties were assessed with SoftEdge Myocam system (IonOptix, Milton, MA, USA). The changes of cardiomyocyte length were monitored using SoftEdge software (14 (link)) and evaluated through the indices as follows: peak shortening (PS), time-to-PS (TPS), time-to-90% re-lengthening (TR90), maximal velocity of shortening (+dL/dt), and re-lengthening (-dL/dt).

Free full text: Click here

Zhu P., Chen Y., Wang J., Lin G., Wang R., Que Y., Zhou J., Xu G., Luo J, & Du Y. (2022). Receptor-Interacting Protein Kinase 3 Suppresses Mitophagy Activation via the Yes-Associated Protein/Transcription Factor EB Pathways in Septic Cardiomyopathy. Frontiers in Cardiovascular Medicine, 9, 856041.

Publication 2022

Sequoia C512 SoftEdge Myocam system

Cardiomyocyte Echocardiography Heart Mice Sequoia Transducer

Corresponding Organization : Chinese PLA General Hospital

Other organizations : Guangzhou University of Chinese Medicine

Top 5 similar protocols

Variable analysis

independent variables

LPS injection

dependent variables

Peak shortening (PS)
Time-to-PS (TPS)
Time-to-90% re-lengthening (TR90)
Maximal velocity of shortening (+dL/dt)
Maximal velocity of re-lengthening (-dL/dt)

control variables

Echocardiography technique (14.0 MHz transducer, Sequoia C512; Acuson, Siemens, Erlangen, Germany)
Cardiomyocyte mechanical property assessment (SoftEdge Myocam system, IonOptix, Milton, MA, USA)
Cardiomyocyte length changes monitoring (SoftEdge software)

controls

No explicit mention of positive or negative controls

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!