Assessment of Metabolic Syndrome and PPAR-γ Polymorphisms

Any case or control participant with dermatological diseases other than psoriasis, polycystic ovarian syndrome, coronary artery disease, breast cancer, and/or blood transfusion in the last 6 months was excluded from the study.
Every case and control participant underwent the following steps

Determination of BMI.[34 (link)] Selected cases and controls included obese and nonobese participants

Evaluation for the presence of MS whose criteria were identified according to the National Cholesterol Education Program Adult Treatment Panel III[35 (link)]

Detection of PPAR-γ gene polymorphism by restriction fragment length polymorphism polymerase chain reaction (PCR).

DNA extraction from the whole blood was done using the GeneJET whole blood genomic DNA purification Mini Kit (Thermo Scientific Lithuania). DNA eluted in buffer AE was stored at −20°C for further PCR procedure.

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Seleit I., Bakry O.A., Abd El Gayed E, & Ghanem M. (2019). Peroxisome Proliferator-Activated Receptor-γ Gene Polymorphism in Psoriasis and Its Relation to Obesity, Metabolic Syndrome, and Narrowband Ultraviolet B Response: A Case–Control Study in Egyptian Patients. Indian Journal of Dermatology, 64(3), 192-200.

Publication 2019

GeneJET whole blood genomic DNA purification Mini Kit

Adult Blood Blood transfusion Breast cancer Buffer Cholesterol Coronary artery disease Dermatological diseases Education program Genomic Obese Polycystic ovarian syndrome Polymerase chain reaction Polymorphism Ppar γ Psoriasis Restriction fragment length polymorphism

Corresponding Organization :

Other organizations : Menoufia University

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Variable analysis

independent variables

PPAR-γ gene polymorphism

dependent variables

Presence of metabolic syndrome (MS)

control variables

Dermatological diseases other than psoriasis, polycystic ovarian syndrome, coronary artery disease, breast cancer, and/or blood transfusion in the last 6 months
Body Mass Index (BMI) (obese and non-obese participants)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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