Tumor-bearing mice were each injected with 5–10 MBq of 64Cu-NOTAMSN-PEG-VEGF121 or 64Cu-NOTA-MSN-PEG via tail vein before serial PET scans. PET scans on microPET/microCT Inveon rodent model scanner (Siemens Medical Solutions USA, Inc.), image reconstruction and ROI analysis of the PET data were performed using described previously procedures.33 (link) Quantitative PET data was presented as percentage injected dose per gram of tissue (%ID/g).
After the last time point at 22 h p.i., mice were euthanized and biodistribution studies were carried out to validate the %ID/g values and radioactivity distribution based on PET imaging in tumor-bearing mice. Blood, U87MG tumor, and major organs/tissues were collected and wet-weighed. The radioactivity in the tissues was measured using a gamma-counter (Perkin-Elmer) and presented as %ID/g (mean ± SD).
After the last time point at 22 h p.i., mice were euthanized and biodistribution studies were carried out to validate the %ID/g values and radioactivity distribution based on PET imaging in tumor-bearing mice. Blood, U87MG tumor, and major organs/tissues were collected and wet-weighed. The radioactivity in the tissues was measured using a gamma-counter (Perkin-Elmer) and presented as %ID/g (mean ± SD).
