Comprehensive Immunohistochemistry Profiling

Immunohistochemistry was performed as previously described (17 (link)). A pressure cooker (95°C for 30 min followed by 120°C for 10 s) was used for antigen retrieval using Antigen Unmarking Solution (Vector Laboratories). Antibodies specific to CHD1 (Sigma, #HPA022236), AKT-473P (Cell Signaling, #4060s), Keratin 5 (BioLegend 905501), Cytokeratin-8 (BioLegend 904801), CD8 (Bioss bs-0648R), Ly6G (BioLegend 127601), CD15 (DAKO M3631), IL‑6R (R&D Systems AF1830-SP) and Ki67 (Thermo Scientific RM 9106-S1) were purchased. TUNEL staining was performed using the TUNEL Assay Kit (Abcam ab206386) according to manufacturer’s instructions. Slides were scanned using Pannoramic 250 Flash III (3DHISTECH Ltd) and images were captured through Pannoramic Viewer software (3DHISTECH Ltd). Human prostate hyperplasia and cancer tissue array samples were purchased from US Biomax (PR753a).

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Zhao D., Cai L., Lu X., Liang X., Li J., Chen P., Ittmann M., Shang X., Jiang S., Li H., Meng C., Flores I., Song J.H., Horner J.W., Lan Z., Wu C.J., Li J., Chang Q., Chen K.C., Wang G., Deng P., Spring D.J., Wang Y.A, & DePinho R.A. (2020). Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer. Cancer discovery, 10(9), 1374-1387.

Publication 2020

Antigen Unmarking Solution Keratin 5 Cytokeratin-8 IL‑6R TUNEL Assay Kit Pannoramic 250 Flash III Pannoramic Viewer software

Antibodies Antigen Assay Cancer Chd1 Cytokeratin 8 Human Il 6r Immunohistochemistry Keratin 5 Pressure Prostate hyperplasia Tissue Tunel Vector

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, University of Notre Dame, Baylor College of Medicine

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Variable analysis

independent variables

Antigen Retrieval Conditions (95°C for 30 min followed by 120°C for 10 s)

dependent variables

Immunohistochemical staining for CHD1, AKT-473P, Keratin 5, Cytokeratin-8, CD8, Ly6G, CD15, IL-6R, and Ki67
TUNEL staining

control variables

Tissue Samples (Human prostate hyperplasia and cancer tissue array samples)

controls

Positive Control: None specified
Negative Control: None specified

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