Single-Cell RNA-Seq Analysis of Periodontitis

scRNA-seq was performed in a subset of the gingival tissue samples harvested. Specifically, periodontitis-affected and healthy gingival samples (one each) from three periodontitis patients were sequenced using a 10× Genomics platform (10× Genomics, San Francisco, CA, USA) as described.^{13 (link)} In addition, the gene raw counts and normalized gene expression matrix for previously reported single-cell analysis of periodontitis-affected and healthy gingival samples from two periodontitis patients and two periodontally healthy subjects, respectively, were downloaded from the GEO database (https://www.ncbi.nlm.nih.gov/geo/).^{14 (link)}