Sections were permeabilized and blocked as described previously15 (link). Anti-col1a1 (AB_2904565, Cell Signaling Technology), anti-laminin (AB_10001146, Novus), anti-CD45R/B220 (AB_2896201, eBioscience) for B cells, and dapi or NucSpot Live 488 (Biotium) were used for nuclear staining. Stained samples were mounted in SlowFade Glass (Invitrogen). Micrographs were acquired on a Leica Thunder TIRF instrument in epifluorescence (Leica DMI8), using 20x objective (Leica HC PL APO 20x/0.80 DRY). Laminin fluorescence was corrected for flatness of field using a dyed slide reference and stitched using LAS-X (LAS X 3.7.5.24914).
For sequential staining of sections, initial immunostaining and imaging was performed as described, the samples decoverslipped, and then stained for Masson’s trichrome
For sequential staining of sections, initial immunostaining and imaging was performed as described, the samples decoverslipped, and then stained for Masson’s trichrome
