Protocol detail

Platelet Aggregation Assay Protocol

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For aggregation assays, platelets in plasma was prepared and platelet counts were normalized (300,000/µL) using a Hemavet 950 (Drew Scientific, Waterbury, CT). Collagen (3 µg/ml) was monitored by light transmittance (Chrono-log Corp., Havertown, PA)13 (link)17 (link).

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Ziu E., Hadden C., Li Y., Lowery CL I.I.I., Singh P., Ucer S.S., Mercado C.P., Gu H.H, & Kilic F. (2014). Effect of serotonin on platelet function in cocaine exposed blood. Scientific Reports, 4, 5945.

Publication 2014

Hemavet 950

Assays Collagen Light Plasma Platelet counts Platelets

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Variable analysis

independent variables

Collagen (3 µg/ml)

dependent variables

Platelet aggregation (monitored by light transmittance)

control variables

Platelet counts were normalized (300,000/µL) using a Hemavet 950

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