Mice were sacrificed by decapitation and the brain rapidly removed, hippocampi promptly dissected and homogenized as described [25] (link). Proteins were denatured, reduced and separated on 10% Tris-Glycine SDS-PAGE gels (Anamed, Germany). After transfer, nitrocellulose membranes were probed with primary antibodies specific for protein tau and tubulin as described [25] (link) and for CNPase (Millipore - MAB326) as marker for myelin.
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