Western Blot Analysis of Joint Tissue Lysates

The quantified joint tissue lysates were assessed by Western blot analysis as described previously23 . Briefly, protein lysates were subjected to 10% SDS-PAGE and then electro-transferred onto nitrocellulose membrane. After blocking with 5% non-fat milk in TBST, the membrane was incubated with designated primary antibodies overnight. The membrane was then incubated with anti-mouse or anti-rabbit secondary antibodies48 (link)49 (link). The specific immunoreactive bands were detected using enhanced chemiluminescence ECL detection kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instruction.