Western Blot Antibody Optimization Protocol

Western blot was conducted as previously described (38 (link)) with the primary antibodies: for NF90, ab131004, 1:2,000, Abcam; for EZH2, #07-689, 1:2,000, Millipore; for NRAS, ab154291, 1:1,000, Abcam; for GAPDH, ab8245, 1:5,000, Abcam; for phospho-MEK1/2, #9154, 1:1,000, Cell Signaling Technology; for MEK1/2, #8727, 1:1,000, Cell Signaling Technology; for phospho-ERK1/2, #4370, 1:2,000, Cell Signaling Technology; for ERK1/2, #4695, 1:1,000, Cell Signaling Technology.

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Chen X.J., Liu S., Han D.M., Han D.Z., Sun W.J., Zhao X.C., Liang J.Q, & Yu L. (2021). FUT8-AS1 Inhibits the Malignancy of Melanoma Through Promoting miR-145-5p Biogenesis and Suppressing NRAS/MAPK Signaling. Frontiers in Oncology, 10, 586085.

Publication 2020

ab131004 ab154291 ab8245 phospho-MEK1/2 MEK1/2 phospho-ERK1/2 ERK1/2

Antibodies Erk1 Ezh2 Gapdh Mek1 Nras Western blot

Corresponding Organization :

Other organizations : Inner Mongolia Medical University, 82th Hospital of Pla

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Variable analysis

independent variables

Antibodies used for Western blot analysis

dependent variables

Protein expression levels of NF90, EZH2, NRAS, GAPDH, phospho-MEK1/2, MEK1/2, phospho-ERK1/2, and ERK1/2

control variables

Western blot protocol used as previously described (38)

controls

No positive or negative controls were explicitly mentioned in the information provided.

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