SRPX2 Gene Silencing Protocol

Three siRNAs targeting SRPX2 (named si-SRPX2_001, si-SRPX2_002, and si-SRPX2_003, respectively) and a nontargeting control siRNA (named scramble siRNA) were purchased from RiboBio (Guangdong, China). The specific SRPX2-targeted siRNA sequences were as follows: si-SRPX2_001: 5'-CAG ATG AAA GCT ACA ATG A-3', si-SRPX2_002: 5'-CAG ATG AAA GCT ACA ATG A-3', and si-SRPX2_3: 5'-GAG GAA ATC TTC ACA TTC A-3'. For transfection, Lipofectamine 3000 (Invitrogen, CA, USA) was used according to previously reported 18 (link).

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Wang Q., Liu J., Hu Y., Pan T., Xu Y., Yu J., Xiong W., Zhou Q, & Wang Y. (2021). Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition. Theranostics, 11(14), 7110-7125.

Publication 2021

scramble siRNA Lipofectamine 3000

Lipofectamine Sirna Srpx2 Transfection

Corresponding Organization :

Other organizations : Tongji Hospital, China-Japan Friendship Hospital, Shanghai Ninth People's Hospital, Huazhong University of Science and Technology

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Variable analysis

independent variables

Si-SRPX2_001
Si-SRPX2_002
Si-SRPX2_003

dependent variables

Not explicitly mentioned

control variables

Lipofectamine 3000 transfection method
Scramble siRNA (nontargeting control)

Annotations

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