Total RNA Extraction and qRT-PCR Analysis

Total RNA was extracted from cells using miRNeasy (Qiagen, Valencia, CA) as previously described [57 (link)]. Equal amounts of RNA were reverse transcribed into cDNA using the miScript II RT Kit and qRT-PCR was conducted with miScript SYBR Green (Qiagen). mRNA expression was normalized to beta actin, while small RNA expression was normalized to RNU6B (RNU6B_13, Qiagen). The polyA site was identified in BCAR3 from PEO4 and 2008 cells using Thermoscript RT (Invitrogen) and oligo dT as previously described [58 ]. PCR products were amplified using the 5’ forward BCAR3 primer with the Universal reverse primer from Qiagen and amplified using GoTaq Green (Promega, Madison, WI). Primers are listed in Supplementary Table 3. PCR products were cloned into T-vector (Life Technologies, Carlsbad, CA) and sent for direct sequencing (University of Minnesota Genomic Center, Minneapolis, MN).