Preparation of B[a]P Dispersion Medium

B[a]P was purchased from (A2385, Sigma Aldrich Co., LLC, St. Louis, MO, USA), dissolved in a modified dispersion medium (DM) of 5.5 mM D-glucose and 0.01 mg/l 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in Ca²⁺ and Mg²⁺-free phosphate buffer saline (PBS), sonicated by a bath-type sonicator, and stored in a refrigerator at 4 °C. Bovine serum albumin (BSA) was removed from the original DM, which was developed for the dispersion of nanoparticles and described previously [34 (link),35 (link)], to prepare the modified DM to optimize the dispersion of B[a]P.

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Abd El Naby W.S., Zong C., Fergany A., Ekuban F.A., Ahmed S., Reda Y., Sato H., Ichihara S., Kubota N., Yanagita S, & Ichihara G. (2023). Exposure to Benzo[a]pyrene Decreases Noradrenergic and Serotonergic Axons in Hippocampus of Mouse Brain. International Journal of Molecular Sciences, 24(12), 9895.

Publication 2023

A2385

Bath Bovine serum albumin Buffer Glucose Phosphate Saline Sn glycero 3 phosphocholine

Corresponding Organization : Tokyo University of Science

Other organizations : Alexandria University, University of Louisville, Jichi Medical University

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Variable analysis

independent variables

Removal of bovine serum albumin (BSA) from the original dispersion medium (DM)

dependent variables

Optimization of the dispersion of B[a]P

control variables

Concentration of D-glucose (5.5 mM) in the modified DM
Concentration of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (0.01 mg/l) in the modified DM
Use of Ca2+ and Mg2+-free phosphate buffer saline (PBS) in the modified DM
Sonication of the B[a]P solution using a bath-type sonicator
Storage of the B[a]P solution in a refrigerator at 4 °C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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