Site-Specific Protein Modification Protocols

Unless otherwise noted, all chemicals
and solvents were of analytical grade and received from commercial
sources. Water (dd-H₂O) used in biological procedures and
as a reaction solvent was deionized using a Barnstead NANOpure purification
system (ThermoFisher, Waltham, MA). Oligonucleotides were purchased
from Integrated DNA Technologies (Coralville, IA) and MilliporeSigma
(Burlington, MA). The pDule-para-aminoPhe (Addgene
#85502),^{36 (link)} pDule-3-nitroTyrosine (Addgene
#85498),^{50 (link)} and pBad-sfGFP 150TAG (Addgene
#85483)^{51 (link)} plasmids were gifts from Ryan
Mehl. Amicon Ultra MWCO centrifugal concentrators were obtained from
MilliporeSigma (St. Louis, MO).

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Bischoff A.J., Hamerlynck L.M., Li A.J., Roberts T.D., Ginsberg N.S, & Francis M.B. (2023). Protein-Based Model for Energy Transfer between Photosynthetic Light-Harvesting Complexes Is Constructed Using a Direct Protein–Protein Conjugation Strategy. Journal of the American Chemical Society, 145(29), 15827-15837.

Publication 2023

Barnstead NANOpure purificationsystem Oligonucleotides

Biological Gifts Nitrotyrosine Oligonucleotides Plasmids Solvent

Corresponding Organization : Lawrence Berkeley National Laboratory

Other organizations : Kavli Energy NanoScience Institute

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Variable analysis

independent variables

Chemicals and solvents used
Water purification method
Oligonucleotide sources
Plasmids used (pDule-para-aminoPhe, pDule-3-nitroTyrosine, pBad-sfGFP 150TAG)

dependent variables

Not explicitly mentioned

control variables

Analytical grade chemicals and solvents used
Deionized water (dd-H2O) used in biological procedures and as a reaction solvent
Amicon Ultra MWCO centrifugal concentrators used

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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