Characterization of DUSP26-N (C152S) Protein

After system equilibration in 50 mM Bis-Tris (pH 6.0), 1 mM DTT at RT, the samples were analyzed with three detectors in series, namely, the UV and light-scattering detectors of the DAWM HELEOS II system (Wyatt Technology) coupled to a refractive-index detector (Optilab T-rEX refractometer; all from Wyatt Technology Corp., Wyatt Technology Europe GmbH, In der Steubach, Germany). As previously described [42 (link)–44 (link)], analysis was performed at RT by injecting the DUSP26-N (C152S) protein sample of 100 μL (2.5 mg/mL) into the SEC-MALS system (WTC-015S5 column, Wyatt Technology) at Korea Basic Science Institute in a mobile phase consisting of 50 mM Bis-Tris (pH 6.0) and 1 mM DTT at a flow rate of 0.5 mL/min. Data were analyzed and weight-averaged molar masses were calculated using ASTRA software (V6, Wyatt Technology).