Genetic and Injury Models of Kidney Disease

The proximal tubule-specific DabA-L-deletion mice were generated by crossing DsbA-L (flox/flox) mice (provided by Dr. Feng Liu Lab) with PEPCK-Cre mice (provided by Jackson Laboratory) as previously described^{35 (link)}. The UUO model was established by ligating the left ureter in mice, also as previously described^{11 (link),41 (link),42} . For ischemic acute kidney injury, it was induced by the duration of bilateral clamping for 28 min and followed by reperfusion^{43 (link)}. For cisplatin injury, mice were intraperitoneally injected with 10 mg/kg cisplatin at weeks 0, 1, and 3⁴⁴ . For aristolochic acid injury, mice were intraperitoneally injected with 250 mg/kg aristolochic acid^{45 (link)}. Hsp90β siRNA was administered twice a week by tail vein injection in C57BL/6 mice at a dose of 15 mg/kg, with saline as a control. Animal experiments were performed in accordance with guidelines approved by the Animal Care Ethics Committee of Second Xiangya Hospital, People’s Republic of China, and ethical approval was obtained. Mice were housed in a 12-h light/dark cycle with free access to a standard rodent diet and water.