Embryo Elongation Rescue via Targeted Kinase Inhibition
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Corresponding Organization : Children's Hospital of Philadelphia
Other organizations : University of York, Lurie Children's Hospital, Northwestern University, University of East Anglia, Norwich Research Park, University of Iowa Hospitals and Clinics, Ambry Genetics (United States), University of Colorado Anschutz Medical Campus, Maine Medical Center, St. Louis Children's Hospital, Capital Medical University, Beijing Children’s Hospital, Xinjiang Medical University, First Affiliated Hospital of Xinjiang Medical University, Harvard University, Guangxi Maternal and Child Health Hospital, Boston Children's Hospital, Columbia University Irving Medical Center, Klinikum rechts der Isar, Technical University of Munich, Ludwig-Maximilians-Universität München, University of Alabama at Birmingham, Université Paris Cité, Assistance Publique – Hôpitaux de Paris, Sorbonne Université, Birmingham Women’s and Children’s NHS Foundation Trust, University Medical Center Hamburg-Eppendorf, Universität Hamburg, Trillium Health Centre, Université de Bourgogne, Inserm, Université Bourgogne Franche-Comté, Addenbrooke's Hospital, University of Cambridge, University of North Carolina at Chapel Hill, McMaster University, Radboud University Nijmegen, Radboud University Medical Center, Istituto Giannina Gaslini, Istituti di Ricovero e Cura a Carattere Scientifico, McGill University Health Centre, McGill University, University of L'Aquila
Variable analysis
- PF06260933 concentration (1 μM, 5 μM, or 10 μM)
- PD0325901 concentration (1 μM)
- SU5402 concentration (5 μM)
- Embryo elongation
- Time of drug application (from 4.5 hpf until imaging)
- Positive control: Embryos treated with PD0325901 (1 μM) or SU5402 (5 μM) to rescue elongation caused by MEK inhibition
- Negative control: Embryos treated with an equivalent amount of DMSO
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