1 mM of Lat-A stock (L12370, Thermo Fisher Scientific) was prepared by reconstituting the chemical in DMSO. The embryos were treated with Lat-A at a final concentration of 2 nM. 1 mM of Blebbistatin stock (13013–5, Enco) was prepared by reconstituting the chemical in DMSO. The embryos were treated with Blebbistatin at a final concentration of 2 µM. For the double inhibition experiments, embryos were treated with Blebb at a final concentration of 1.5 µM, and with Lat-A at a final concentration of 2 nM.
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