Engineered Yeast Strains for Cell Cycle Study

All strains were constructed using homologous recombination in a prototrophic Saccharomyces cerevisiae W303 derived background. Plasmid containing NTH1 were constructed using standard molecular cloning. Plasmids were linearized with either SalI in the promoter (full-length constructs) and integrated into the genomic NTH1 promotor or with StuI in the URA3 region (reporter constructs) and integrated into the URA3 locus. All constructs were Sanger-sequenced. Cell cycle inducible strains from our previous study^{16 (link)} were used. Estradiol inducible strains were transformed and maintained on YPD plates containing 100 nM β-estradiol (Alfa Aesar). A detailed strain list is included in the Supplementary Information.