De novo BPDCN Mouse Model

Male NOD SCID Gamma (NSG) mice at 7 to 8 weeks old purchased from The Jackson Laboratory were used for CAL-1 xenograft experiments. TET2 ^Δ/Δ P53 ^Δ/Δ DKO + MYC RUNX2 OE, a de novo BPDCN mouse model was generated by Kubota and colleagues (2019).^{5 (link)} RNA-sequencing (RNA-seq) data obtained from leukemic cells from this mouse model were used for gene expression analyses.

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Rethnam M., Tan D.Q., Tan S.H., Li J., Yokomori R., Li Y., Yang H., Sanda T, & Suda T. (2022). Loss of METTL3 attenuates blastic plasmacytoid dendritic cell neoplasm response to PRMT5 inhibition via IFN signaling. Blood Advances, 6(18), 5330-5344.

Publication 2022

Male NOD SCID Gamma (NSG) mice

Cells Gamma Gene expression analyses Male Mouse Nod mice Runx2 Scid mice Xenograft

Corresponding Organization :

Other organizations : National University Cancer Institute, Singapore, National University of Singapore, Kumamoto Medical Center

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Variable analysis

independent variables

TET2 Δ/Δ P53 Δ/Δ DKO
MYC RUNX2 OE

dependent variables

Gene expression in leukemic cells

control variables

Male NOD SCID Gamma (NSG) mice at 7 to 8 weeks old purchased from The Jackson Laboratory

controls

The de novo BPDCN mouse model (TET2 Δ/Δ P53 Δ/Δ DKO + MYC RUNX2 OE) was used as a positive control to generate leukemic cells for gene expression analysis.

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