Characterization of CatSper Antibodies

In-house rabbit polyclonal CatSper1 (Ren et al., 2001 (link)), CatSper3 (Qi et al., 2007 (link)), CatSperε (Chung et al., 2017 (link)) antibodies were described previously. Polyclonal CA-IV antibody (M-50) was purchased from Santacruz. Monoclonal antibodies were purchased from BD Biosciences: anti-caveolin1 (clone 2297); EMD Milipore: anti-phosphotyrosine (clon4G10), anti-acetylated tubulin (clone 6-11B-1), anti-HA agarose (clone HA-7); Thermo Scientific: anti-HA magnetic beads; and Cell Signaling Technology: β-actin (clone 13E5) and HA (clone C29F4). HRP-conjugated goat anti-rabbit IgG and goat anti-mouse IgG were from Jackson Immunoresearch. PNA-Alexa 568, WGA-Alexa 555, WGA-Alexa 647, goat anti-mouse IgG (Alexa 488 or 647), and goat anti-rabbit IgG (Alexa 568 or Alexa 647) were from Invitrogen. H89, calyculin A, and calpain inhibitor I were purchased from Calbiochem. ST-Ht31 was from Promega. Calpain inhibitor II and III were from Enzo life science. All other chemicals were from Sigma-Aldrich unless indicated.

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Ded L., Hwang J.Y., Miki K., Shi H.F, & Chung J.J. (2020). 3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice. eLife, 9, e62043.

Publication 2020

anti-HA magnetic beads β-actin (clone 13E5) HA (clone C29F4). HRP-conjugated goat anti-rabbit IgG goat anti-mouse IgG WGA-Alexa 555 goat anti-rabbit IgG (Alexa 568 ST-Ht31

Actin Agarose Alexa 568 Anti igg Antibodies Calpain ii Calpain inhibitor i Calyculin a Clone Goat House rabbit Ht31 Iv antibody Monoclonal antibodies Mouse Phosphotyrosine Promega Rabbit Tubulin

Corresponding Organization :

Other organizations : Yale University, Czech Academy of Sciences, Institute of Biotechnology, Boston Children's Museum, Boston Children's Hospital

Top 5 similar protocols

Variable analysis

independent variables

Calyculin A
Calpain inhibitor I
ST-Ht31
Calpain inhibitor II
Calpain inhibitor III

dependent variables

CatSper1 expression
CatSper3 expression
CatSperε expression
Caveolin1 expression
Phosphotyrosine levels
Acetylated tubulin levels
HA tag expression

control variables

Polyclonal CatSper1, CatSper3, and CatSperε antibodies
Polyclonal CA-IV antibody (M-50)
Monoclonal antibodies: anti-caveolin1, anti-phosphotyrosine, anti-acetylated tubulin, anti-HA agarose, anti-HA magnetic beads, β-actin, and HA
HRP-conjugated goat anti-rabbit IgG and goat anti-mouse IgG
PNA-Alexa 568, WGA-Alexa 555, WGA-Alexa 647, goat anti-mouse IgG (Alexa 488 or 647), and goat anti-rabbit IgG (Alexa 568 or Alexa 647)

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