HIV Antibody Detection and Viral Load Quantification

Blood samples were tested for HIV antibodies by AxSYM HIV-1/2 gO (Abbott) and confirmed by Western Blot (MP Diagnostics). Recent HIV infection was detected by the LAg assay (Sedia™ Biosciences Corporation) (Duong et al., 2015 (link)). HIV-RNA was quantified in HIV positive samples in TRIP using Artus HI Virus-1 RG RT–PCR (Qiagen). HIV-RNA at HIV clinics was measured using Cobas Taqman HIV, PCR RNA, branched DNA 3.0, and Versant HIV-1 RNA 1.0 kPCR.

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Psichogiou M., Giallouros G., Pantavou K., Pavlitina E., Papadopoulou M., Williams L.D., Hadjikou A., Kakalou E., Skoutelis A., Protopapas K., Antoniadou A., Boulmetis G., Paraskevis D., Hatzakis A., Friedman S.R, & Nikolopoulos G.K. (2019). Identifying, linking, and treating people who inject drugs and were recently infected with HIV in the context of a network-based intervention. AIDS care, 31(11), 1376-1383.

Publication 2019

AxSYM HIV-1/2 gO Artus HI Virus-1 RG RT–PCR

Assay Blood Diagnostics Hiv 1 Hiv 2 Hiv antibodies Hiv infection Rt pcr Virus Western blot

Corresponding Organization : University of Cyprus

Other organizations : National and Kapodistrian University of Athens, Laiko General Hospital of Athens, National Development and Research Institutes, European University Cyprus, Evangelismos Hospital, University General Hospital Attikon, Hospital Agioi Anargyroi

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

HIV antibodies
Recent HIV infection
HIV-RNA

control variables

None explicitly mentioned

controls

Positive control: Western Blot (MP Diagnostics) used to confirm HIV antibodies
Negative control: Not specified

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