Preparation of Viscum album Dry Extract

V. album hydroalcoholic extract was obtained by maceration at room temperature followed by a daily agitation for 21 days, according to the methodology used by Holandino et al. [22 (link)] and described in the French and Brazilian Homeopathic Pharmacopoeias [44 ,45 ]. The plant material was fragmented and submitted to the total volume of the solvent in an environment protected from direct action of light and heat. After the maceration period, the extract was filtered. At last, the solvent was concentrated in a rotary evaporator (Büchi, Vacuum Pump V-700) under vacuum (Büchi 461 Water Bath) at 40 °C. The extract was frozen and subsequently dried by lyophilization (Christ Beta 2-8 LD) to obtain the V. album dry extract (VADE).