Experimental Evolution of Pseudomonas aeruginosa

The strains used in this study were obtained by experimental evolution as previously described [30 (link)] (Tables S1 and S2, available with the online version of this article). Briefly, P. aeruginosa PAO1 biofilms were repeatedly exposed to the QSI C-30 (100 µg ml⁻¹), tobramycin (20 µg ml⁻¹), or a combination of C-30 and tobramycin, in SCFM2, during 16 cycles. P. aeruginosa was maintained on tryptone soy agar (TSA, Neogen) and all overnight cultures were prepared in Lysogeny Broth (LB, Neogen). For each condition three independent replicate populations (lineages) were used. Whole populations of the evolved lineages were used for subsequent analyses.

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Bové M., Kolpen M., Lichtenberg M., Bjarnsholt T, & Coenye T. (2023). Adaptation of Pseudomonas aeruginosa biofilms to tobramycin and the quorum sensing inhibitor C-30 during experimental evolution requires multiple genotypic and phenotypic changes. Microbiology, 169(1), 001278.

Publication 2023

Lysogeny Broth (LB,

Agar Biofilms Evolution Lysogeny P aeruginosa Populations Replicate Strains Tobramycin

Corresponding Organization :

Other organizations : Ghent University, Rigshospitalet, University of Copenhagen

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Variable analysis

independent variables

Exposure of P. aeruginosa PAO1 biofilms to the following treatments:
- QSI C-30 (100 µg ml^-1)
- Tobramycin (20 µg ml^-1)
- Combination of C-30 and tobramycin

dependent variables

Not explicitly mentioned

control variables

P. aeruginosa PAO1 biofilms were maintained on tryptone soy agar (TSA) and all overnight cultures were prepared in Lysogeny Broth (LB)
SCFM2 media was used for the evolution experiments

controls

Three independent replicate populations (lineages) were used for each condition

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