The effective quantum yield of PSII (Y(II); false colored image) and near infrared remission (NIR, 780 nm) were visualised at the subcellular level with the microscopic version of an Imaging-PAM (M-series, Heinz Walz GmbH, Effeltrich, Germany; Herburger and Holzinger 2015 (link)). Therefore, algal filaments of Zygogonium AUT-p and Zygogonium AUT-g exhibiting different cell morphologies (younger or older filaments, different stages of aplanospore formation and pigmentation) were collected by using a stereomicroscope (Lumar V12; Carl Zeiss AG) and transferred to welled slides. A modified Axio Scope A.1 epifluorescence microscope equipped with a Zeiss Fluar 40×1.3 NA objective and CCD Camera IMAG-K6 controlled with ImagingWinGigE (V2.45i) software was used for image generation. Measuring light for Y(II) determination was provided by a LED (620 nm). The signal/noise ratio was not modified by a SP-Routine.
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Subcellular Imaging of Photosynthesis in Zygogonium
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Corresponding Organization : Universität Innsbruck
Other organizations : University of Applied Sciences Upper Austria
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Variable analysis
independent variables
- Cell morphology (younger or older filaments, different stages of aplanospore formation and pigmentation) of Zygogonium AUT-p and Zygogonium AUT-g algal filaments
- Effective quantum yield of PSII (Y(II); false colored image)
- Near infrared remission (NIR, 780 nm)
- Measuring light for Y(II) determination (LED, 620 nm)
- Signal/noise ratio (not modified by a SP-Routine)
- No positive or negative controls were explicitly mentioned in the provided information.
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