Cell culture, quantitative RT-PCR (qPCR), Western blot analyses and immunochemistry were performed according to routine protocols. miR-155 mutant mice and calcineurin transgenic mice (Tg(Myh6-Ppp3ca)37Eno ), which were originally generated by Drs. Molkentin and Olson, were obtained from the Jackson lab. The transverse aortic constriction (TAC) operation and measurement of cardiac function by echocardiography are described as described 9 (link).
Protocol detail
Cardiac Hypertrophy Analysis Protocol
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Aortic constriction
Calcineurin
Cell culture
Echocardiography
Function cardiac measurement
Mice
Ppp3ca
Rt pcr
Transgenic mice
Western blot analyses
Corresponding Organization :
Other organizations : Beijing University of Chinese Medicine, Harvard Stem Cell Institute, Harvard University, Boston Children's Hospital
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Protocol cited in 8 other protocols
Variable analysis
independent variables
- MiR-155 mutant mice
- Calcineurin transgenic mice [Tg(Myh6-Ppp3ca)37Eno]
- Transverse aortic constriction (TAC) operation
- Cardiac function measured by echocardiography
- Cell culture
- Quantitative RT-PCR (qPCR)
- Western blot analyses
- Immunochemistry
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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