Synthesis and Utilization of DT2216 Compound

DT2216 was synthesized in Dr. Guangrong Zheng's laboratory (University of Florida, Gainesville, FL) according to the previously described protocol (20 (link)). Gemcitabine (catalog No. S1714), 5FU (catalog No. S1209), niraparib (catalog No. S2741), A1155463 (catalog No. S7800), ABT199 (catalog No. S8048), S63845 (catalog No. S8383), and ABT263 (catalog No. S1001) were purchased from SelleckChem. All the compounds were dissolved in DMSO at 10 mmol/L stock solution for in vitro assays.

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Thummuri D., Khan S., Underwood P.W., Zhang P., Wiegand J., Zhang X., Budamagunta V., Sobh A., Tagmount A., Loguinov A., Riner A.N., Akki A.S., Williamson E., Hromas R., Vulpe C.D., Zheng G., Trevino J.G, & Zhou D. (2021). Overcoming Gemcitabine Resistance in Pancreatic Cancer Using the BCL-XL–Specific Degrader DT2216. Molecular Cancer Therapeutics, 21(1), 184-192.

Publication 2021

Gemcitabine niraparib A1155463 ABT199 S63845 ABT263

Abt199 Abt263 Assays Dmso Dt2216 Gemcitabine Niraparib S63845

Corresponding Organization : University of Florida

Other organizations : The University of Texas Health Science Center at San Antonio, Virginia Commonwealth University

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Variable analysis

independent variables

DT2216 synthesized in Dr. Guangrong Zheng's laboratory (University of Florida, Gainesville, FL) according to the previously described protocol (20 (link))
Gemcitabine (catalog No. S1714)
5FU (catalog No. S1209)
Niraparib (catalog No. S2741)
A1155463 (catalog No. S7800)
ABT199 (catalog No. S8048)
S63845 (catalog No. S8383)
ABT263 (catalog No. S1001)

dependent variables

Not explicitly mentioned

control variables

All the compounds were dissolved in DMSO at 10 mmol/L stock solution for in vitro assays

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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