Antimicrobial Activity Screening Protocol

The antimicrobial activity was tested on Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Listeria inoccua ATCC 33096, Listeria ivanovii ATCC 19110), and Gram-negative (Escherichia coli ATCC 25922, Salmonella typhimurium vs. enterica ATCC 14028, Shigella flexneri ATCC 12022) bacterial as well as yeast (Candida glabrata ATCC MYA-2950, Saccharomyces cerevisiae SMR4) reference strains.
Four species of fungi, Aspergillus niger, Alternaria alternata, Rhizopus nigricans, and Cladosporium cladosporioides strains, were obtained from the Microbial Strain Collection of Faculty of Biology, University of Bucharest, Romania, and confirmed by MALDI-TOF.

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Marinas I.C., Oprea E., Gaboreanu D.M., Gradisteanu Pircalabioru G., Buleandra M., Nagoda E., Badea I.A, & Chifiriuc M.C. (2023). Chemical and Biological Studies of Achillea setacea Herba Essential Oil—First Report on Some Antimicrobial and Antipathogenic Features. Antibiotics, 12(2), 371.

Publication 2023

Alternaria alternata Antimicrobial Aspergillus niger Bacillus cereus Bacterial Candida glabrata Cladosporium cladosporioides Escherichia coli Faculty Fungi Listeria Listeria ivanovii Maldi Rhizopus nigricans Saccharomyces cerevisiae Salmonella typhimurium Shigella flexneri Staphylococcus aureus Strain

Corresponding Organization : University of Bucharest

Other organizations : Academy of Romanian Scientists, Romanian Academy

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Variable analysis

independent variables

Antimicrobial activity

dependent variables

Growth of Gram-positive bacterial strains (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Listeria inoccua ATCC 33096, Listeria ivanovii ATCC 19110)
Growth of Gram-negative bacterial strains (Escherichia coli ATCC 25922, Salmonella typhimurium vs. enterica ATCC 14028, Shigella flexneri ATCC 12022)
Growth of yeast strains (Candida glabrata ATCC MYA-2950, Saccharomyces cerevisiae SMR4)
Growth of fungal strains (Aspergillus niger, Alternaria alternata, Rhizopus nigricans, Cladosporium cladosporioides)

control variables

Bacterial and fungal reference strains used

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