This study is focusing on the semi-domesticated cats residing in monasteries, in Bangkok metropolitan, the capital of Thailand. Stratified randomization was conducted by selecting 43 monasteries and 24 districts in three zones for the sampling areas. Population size was calculated according to the Taro Yamane method [24 ] and 500 samples were collected from this study. Two to three monasteries were randomly selected in each district, and five to ten cats were sampled in each monastery. Animal health was examined by a qualified veterinarians prior to sample collection. The form of consent was informed to the animal’s caretakers before sample and data collection. Fecal samples were collected from the rectum, it was immediately placed in a dry bag and stored at 4 °C before transportation to the Department of parasitology, Faculty of Veterinary Medicine Kasetsart University, Bangkok before DNA extraction. A questionnaire was designed to collect animal information on age, sex, free-roaming, veterinary attention, previously deworming and Bangkok zone by interviewing the monks or animal caretakers during sample collection.
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